Heparin is a 10-12 kDa polysaccharide produced in mast cells. Heparin inhibits blood coagulation through binding and activation of anti-thrombin [1] and acts as a cofactor to basic Fibroblast Growth Factor-2 (bFGF) [2, 3, 4]. The effect of heparin on cell growth and morphology in vitro is cell-type dependent and may promote or inhibit cell proliferation. Culture media may be supplemented with heparin solution (HEP) at 1:1000-1:100 (0.4 – 4.0 U/mL). The optimal concentration should be determined for individual cell types and culture conditions.
