pUCSVH388-2 [molecular clone of Simian virus 40, SV-40]
商品貨號
B245003
Designations
pUCSVH388-2 [molecular clone of Simian virus 40, SV-40]
Species
Simian virus 40, Simian virus 40
Depositors
JA Lednicky, JS Butel
Isolation
Description of cloned agent:
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
Applications
contains sequence
Vector
Construct size (kb): 7.914999961853027
DESCRIPTION OF VECTOR COMPONENT
??????? Type of vector:? E. coli
??????? Name of vector:? pUC-19
??????? Intact vector size (kb):? 2.686 kb
Insert
DNA: genomic
Insert lengths(kb): 5.229000091552734
Tissue: monkey brain
Insert Size (kb)
5.229
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Description of cloned agent:
Name: SV40
Strain: H388, variant #2
Classification: Papovavirus
Contains a full-length non-archetypal SV40-H388 genome cloned into pUC-19 at EcoRI site; isolated from brain tissue (animal H388). Plasmid size: 7915 bp [pUC-19 (vector): 2686 bp + SV40-H388 (insert): 5229 bp]. High-copy number; pUC-19 has a ColEI ori.
VRMC-10 (variant #2) is one of a matched set of 3 clones of regulatory region variants (see also VRMC-11 and VRMC-9).
Sequence (viral regulatory region, T-ag carboxy terminus, and part of VP1 gene) determined by J. Lednicky. Viable clone; verif. by lipofection of EcoRI-linearized DNA into CV-1 cells (ATCC CCL-70, passage 35) by J. Lednicky (lipofection date: 1/12/97).
Classification
Polyomaviridae, Polyomavirus
References
Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685
