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Adult Melanocyte Growth Kit
Adult Melanocyte Growth Kit
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貨期:
編號:B244961
品牌:Mingzhoubio

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    產品名稱 Adult Melanocyte Growth Kit
    商品貨號 B244961
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Product Format frozen 1 Kit
    Storage Conditions -20°C (or -70°C for long-term storage)
    Comments The Adult Melanocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete low-serum culture environment for adult melanocytes derived from normal human epidermis (e.g., Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013). The complete low serum medium is formulated to support robust adult melanocyte growth in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). No feeder layers, extracellular matrix proteins or other substrares are required.

    The final concentration for each component in complete adult melainocyte growth medium is as follows:
    • rh Insulin: 5 µg/mL
    • Ascorbic Acid: 50 µg/mL
    • L-Glutamine: 6 mM
    • Epinephrine: 1.0 µM
    • Calcium chloride: 1.5 mM
    • Peptide Growth Factor: 0.2%, Proprietary formulation
    • M8 Supplement: 1%, Proprietary formulation
    Complete Growth Medium
    1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
    2. Thaw the components of the growth kit just prior to adding them to the basal medium.
    3. Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage. 
    4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
    5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer. 
    6. Table 1. Adult Melanocyte Growth Kit Components 

      Component

      Volume

      Volume

      Sterility Tests

      Final Concentration

      Functional Tests

      rh Insulin

      C of A

      0.5 mL

      梅經理 17280875617 1438578920
      胡經理 13345964880 2438244627
      周經理 17757487661 1296385441
      于經理 18067160830 2088210172
      沈經理 19548299266 2662369050
      李經理 13626845108 972239479
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