| 產品名稱 |
pUN30 [pNN332] |
| 商品貨號 |
B243162 |
| Designations |
pUN30 [pNN332] |
| Depositors |
SJ Elledge |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 5.3000001907348630 Vector: pUN30 (phagemid) Promoters: Promoter lac Construction: pUC18 Marker(s):TRP1,ampR Construct size (kb): 5.300000190734863 Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4 |
| Applications |
YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; KpnI--5.4; HindIII--3.7, 1.7. YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA). |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604
|
| Related Products |
component of:ATCC 87634 component of:ATCC 87637 |
| Shipped |
freeze-dried |
