亚洲综合另类小说色区丨三级特黄60分钟在线观看丨131美女mm爱做爽爽爽视频丨成人无码一区二区三区网站丨免费人成小说在线观看网站

in another host, produces protein dihydrofolate reductase

Construct size (kb): 5.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSV2-neo
Intact vector size: 5.729
Type of vector: plasmid
Vector end: HindIII
Vector end: SmaI
Cloning sites: EcoRI BamHI PvuII PstI HindIII
Polylinker sites:
Construction: SV40, Tn5, pBR322
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: SV40
marker(s): G418R, <-
restriction site: HindIII
promoter: SV40 early, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: U02434

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: hamster, Chinese
Gene symbol: DHFR
Gene name: dihydrofolate reductase
Contains complete coding sequence?: U
Tissue: lung cell line
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: BglII
Insert size (kb): 0.71
Cross references:

Insert lengths(kb): 0.7099999785423279

Tissue: lung cell line

Gene product: dihydrofolate reductase [DHFR]

Target Gene: dihydrofolate reductase

Restriction digests of the clone give the following sizes (kb): BamHI--3.5, 1.7; EcoRI--2.7, 2.3; PstI--1.9, 1.4, 0.95, 0.9; PvuII--4.0, 1.1; HindIII/SmaI--5.2.

Transfected cells amplify DHFR sequences.

Encodes the L22F mutant of DHFR that can serve as a dominant selection marker because it retains catalytic activity and also confers methotrexate (MTX) resistance at low MTX concentrations (125 nM).

Constructed by cloning a HindIII/SmaI fragment containing the gene into pSV2-neo from which neoR had been removed. This plasmid was then cut with SmaI and BglII to remove 18 G nucleotides at the 3' end.

The BglII site was filled in, and ligated to the SmaI site, thereby destroying the original BglII and SmaI sites.

Removing the HindIII/SmaI fragment from pSV2-neo removes all but the 3' 171 bp of neoR.