Restriction digests of the clone give the following sizes (kb): HindIII--13.0; PstI--7.5, 5.5; EcoRI--6.4, 4.3, 2.3; BamHI--uncut; XbaI--uncut. For insertional mutagenesis of bacterial or yeast genes carried in bacteriophage lambda vectors. Derived from pNK81 by replacing 1.050 kb of uncharacterized DNA and the his promoter with 4 tandem repeats of the rrnB transcriptional terminator. Contains the mini-Tn10-LUK insert that consists of the following features: IS10R - nt 63 - promoterless lacZ element starting at codon 8 - nt 3140 - URA3 - nt 4320 - kanR - nt 6023 - IS10L - nt 6090. The order of the major features in this plasmid is: EcoRI - rrnB terminators - Tn10-LUK in hisG - hisD - EcoRI - pMB1 ori - ampR. |
