Restriction digests of the clone give the following sizes (kb): PstI/SalI--2.7, 2.4; EcoRI--3.2, 1.9; EcoRV--4.5, 0.5. Insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.82; EcoRV--1.34, 1.79; PvuI--1.33; SphI--1.40. The insert can be expressed in E. coli in the presence of T7 RNA polymerase and iron-supplemented media. Expression from the Yersinia promoter is induced by growth in iron-limiting conditions in Y. erterocolitica as well as in E. coli. Production of the receptor results in pesticin sensitivity (pstS). |
