ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.8; EcoRI--6.4; BglII--6.4; HindIII--6.4.
The BamHI fragment has been used to construct high-level expression vectors for production of fusion proteins.
The additional glycine and serine residues at the carboxy terminus of the protein do not appear to affect amylase activity.
The vector was derived from pBD64 by replacing the PvuII site with a HindIII site.
Altered from the wild-type sequence by inserting a second BamHI site between the last codon and the stop codon.
The order of the major features in this plasmid is: HindIII - BamHI/amylase/BamHI - EcoRI - kanR/BglII - cmlR.
References
Sloma A, et al. Expression of heterologous DNA using the Bacillus coagulans amylase gene. US Patent 5,171,673 dated Dec 15 1992
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
