Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 0.89; HincII--4.3; HindIII--4.3; PstI--4.3; PvuII--3.0, 1.3. Insert contains BglII, SmaI (2), and BamHI sites. A full-length cDNA clone including 35 nt GC-rich 5'-untranslated and a short, 37 nt 3' untranslated region (not including the stop codon). |
