-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
The HCC1599 cell line was initiated on March 28, 1995 from a 44 year-old White female with a TNM stage IIIA, grade 3, invasive ductal carcinoma.? The cell line took 10 months to establish. Information regarding lymph node metastasis is not available. The cells are poorly differentiated. The cells are negative for expression of Her2-neu and for expression of p53. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
HCC1599 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2), and for cytokeratin 19. DNA isolated from an EBV-transformed lymphoblastoid cell line (HCC1599 BL) from the same patient is available as ATCC CRL-2332D.
Quality Control Specifications
> 100 kb in size by agarose gel electrophoresis
No RNA detected in the agarose gel
A260/A280 ratio ≥ 1.8
Tested and verified for PCR amplification and restriction digestion.
