| 產(chǎn)品名稱(chēng) |
pcDmouseIL-2 [MT-1] |
| 商品貨號(hào) |
B239290 |
| Designations |
pcDmouseIL-2 [MT-1] |
| GenBank Number |
K02292
|
| Species |
Mus musculus, mouse |
| Depositors |
DNAX Research Institute of Molecular and Cellular Biology, Inc., EP Ching, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications |
in another host, produces protein interleukin 2 |
| Vector |
Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il2
Genomic copy number: unique
Gene name: interleukin 2
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3
Strain: C57BL/6
Tissue: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 0.816
Cross references: DNA Seq. Acc.: K02292 Insert lengths(kb): 0.8159999847412109 Tissue: T-cell line LB2-1 (ATCC CRL-8629), Con A-induced Gene product: interleukin 2 [Il2] Target Gene: interleukin 2 |
| Insert Size (kb) |
0.816 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 0.94; PstI--2.9, 1.35; XhoI--3.0, 1.1. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.70; BglII--0.31; HindIII--0.38; PstI--0.04; PvuII--0.20; SacI--0.22; SphI--0.06. Contains the complete coding sequence (nt 49-557). Digestion with XhoI excises the insert plus flanking vector sequence. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Yokota T, et al. Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells. Proc. Natl. Acad. Sci. USA 82: 68-72, 1985. PubMed: 3918306
Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742
Lee FD, et al. cDNA clones coding for polypeptides exhibiting murine interleukin-2 activity. US Patent 4,798,789 dated Jan 17 1989
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
