Tissue: HL-60 cell line (promyelocytic), undifferentiated
Gene product: annexin I (lipocortin I) [ANX1]
Insert Size (kb)
1.440
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
The insert contains the following restriction sites, in nucleotides from the 5' of the published sequence: EcoRI--90; HindIII--916, 1177.
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.44; EcoRI--2.9, 1.44; HindIII--3.88, 0.46; BglI--2.9, 1.44; PstI--4.34.
The insert is a complete cDNA clone.
This construct is unpublished. The sequence is the same as that reported by Wallner et al.
The insert was subcloned from a lambdagt10 library by partial EcoRI digestion, size-selection, and addition of EcoRI/XmnI/BamHI linkers.
