Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain
no
Genotype
MATa ura3-52 his6 leu2 deltaspe2-5::LEU2 [YEp352]
Preceptrol®
no
Mating Type
a
Ploidy
haploid
Comments
unable to synthesize spermidine or spermine
paromomycin-sensitive
requires spermidine or spermine
Medium
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Name of Depositor
H Tabor, CW Tabor
Special Collection
NCRR Contract
Isolation
constructed by transforming strain Y344 (ATCC MYA-989) with YEp352
References
Balasundaram D, et al. Oxygen toxicity in a polyamine-depleted spe2delta mutant of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90: 4693-4697, 1993. PubMed: 8506320
Balasundaram D, et al. Sensitivity of spermidine-deficient Saccharomyces cerevisiae to paromomycin. Antimicrob. Agents Chemother. 43: 1314-1316, 1999. PubMed: 10223963
