| 產(chǎn)品名稱 |
293 c18 |
| 商品貨號(hào) |
B163664 |
| Organism |
Homo sapiens, human |
| Tissue |
kidney |
| Cell Type |
transformed with adenovirus 5 DNA |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 CELLS CONTAIN ADENOVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
fetus |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Derivation |
This line is a derivative of 293 (see ATCC CRL-1573) the cells express EBNA1. |
| Comments |
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 12,14 D16S539: 9 D5S818: 8,9 D7S820: 11,12 THO1: 7,9.3 TPOX: 11 vWA: 16,19 |
| Name of Depositor |
Stanford University |
| Deposited As |
Homo sapiens |
| References |
Murphy AJM, et al. cDNA expression cloning in human cells using the pλDR2 episomal vector system. Methods 4(2):111–131,1992.
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