| 產(chǎn)品名稱(chēng) |
Vaccinia virus ts mutant |
| 商品貨號(hào) |
B163394 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts38 |
| Applications |
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. |
| Biosafety Level |
2 BSL 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This conditional lethal mutant, originally designated plaque isolate no. 6389, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
This conditional lethal mutant, originally designated plaque isolate no. 6389, was gnerated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category C, indicating that it induces rudimentary virus membranes with spicules in foci of viroplasm in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature results in an abrupt cessation of DNA synthesis, described as a "fast stop" phenotype.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses |
| Effect on Host |
Cytopathic effects in culture include enlargement and rounding of cells, followed by cell death and sloughing CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions |
1-3 days at 31 C with 55 CO2 |
| Name of Depositor |
S Dales, R. Condit |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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