Features: insert detection: lacZ' marker(s): ampR, URA3 replicon: pMB1, f1
Applications
YI-type (integrating) shuttle vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
ssDNA-producing plasmid with polylinker in lacZ' and marker selectable in yeast.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771
Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935
