Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; NcoI--2.7, 0.7. pPD100 (ATCC 87222) and pPD101 (ATCC 87223) require that the insert provide a translational initiation signal for expression. pPD100 differs from pPD101 only in the orientation of the MCS. Deposited for research purposes only. Before any commercial use, the depositor must be contacted. A low copy number plasmid using the T7 gene 10 promoter for selective, overexpression of cloned genes in E. coli. |
