| Comments |
The insert contains the following sites separated by (kb) (approximate): BamHI- 0.8- BamHI- 0.6- BamHI- 0.7- HindIII- 2.8- BamHI. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--7.8, 4.0, 3.5, 2.3, 0.9; XhoI--11.6, 7.0; XbaI--18.5; XbaI/AvaI--7.0, 3.5, 2.4, 2.0, 1.7, 0.6; BamHI--16.0, 2.5. The insert includes a continuous open reading frame encoding a basic protein of 654 amino acids and 76 kDa. The mRNA is about 2.2 kb. |
