Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 2.0; HindIII--4.7; BamHI--4.7; PstI--4.7; SalI--4.7. The translocation breakpoint on chromosome 14 in the 2 patients characterized occurred either immediately upstream of Ddelta2-Jdelta1 sequences or within Jdelta1 sequences of the T cell receptor. The insert is derived from sequences adjacent to or involved in translocations in 2 T-ALL patients t(1;14)(p32;q11) and retained on the der(1) chromosome. |
