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產品名稱	1A3
商品貨號	B161857
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue	Spleen
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	BALB/c
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 2 x 10 ⁵ and 1 x 10⁶cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Complete growth medium, 90%; DMSO, 10%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Isotype	IgG1
Name of Depositor	Univ. Texas System, Board of Regents
Deposited As	mouse (B cell); mouse (myeloma)
U.S. Patent Number	4,618,585
References	Chan TS. Hybridoma cell lines producing monoclonal antibodies directed against cervical cancer cells. US Patent 4,618,585 dated Oct 21 1986 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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