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SHP-77

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編號:B161411

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產品名稱	SHP-77
商品貨號	B161411
Organism	Homo sapiens, human
Tissue	lung
Cell Type	epithelial
Product Format	frozen
Morphology	rounded
Culture Properties	suspension and loosely adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	carcinoma; small cell lung cancer
Age	54 years
Gender	male
Ethnicity	Caucasian
Applications	SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients. The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.
Storage Conditions	liquid nitrogen vapor phase
Derivation	It was established in 1977 by Edwin R. Fisher and John D. Paulson. The line was derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung.
Clinical Data	54 years Caucasian male
Antigen Expression	Blood Type O; Rh +; CD56; CD57 (HNK-1,Leu-7)
Genes Expressed	neural cell adhesion molecule (NCAM) NKH-1
Cellular Products	neural cell adhesion molecule (NCAM) NKH-1
Tumorigenic	Yes
Effects	Yes, the cells form tumors in athymic nude mice, and usually grow as circumscribed nodules without evidence of metastases
Comments	The SHP-77 (Shadyside Hospital, Pittsburgh, PA) cell line is a biochemically stable continuously cultured cell line which has retained important features of SCLC. This cell line is an unusual undifferentiated large cell variant of small cell lung carcinoma. It has the morphology of a variant, but the biochemical properties of a classic SCLC. Electron microscopy revealed the presence of gland formation and intracytoplasmic lamellar bodies. The cells have neuroendocrine markers L-dopa decarboxylase and dense core secretory granules. SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients. The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at approximately 4-5 x 10⁵ viable cells/mL. Cells will grow in floating clusters. Some cells may adhere but only the floating cells are transferred. Medium Renewal: Every 3 to 4 days
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
STR Profile	Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 11 D5S818: 12 D7S820: 11 THO1: 7 TPOX: 9,11 vWA: 16
Population Doubling Time	96 hrs
Name of Depositor	AM Koros
Deposited As	Homo sapiens
Year of Origin	1977
References	. Immune-deficient animals. Basel: Karger; 1984. . . J. Exp. Clin. Res. 4: 355-363, 1985. Fisher ER, et al. Comparative histopathologic, histochemical, electron microscopic and tissue culture studies of bronchial carcinoids and oat cell carcinomas of lung. Am. J. Clin. Pathol. 69: 165-172, 1978. PubMed: 204185 Koros AM, et al. Stability and utility of the unique human small cell carcinoma line SHP-77. Cancer Res. 45: 2725-2731, 1985. PubMed: 2985251 Carbone DP, et al. Neural cell adhesion molecule expression and messenger RNA splicing patterns in lung cancer cell lines are correlated with neuroendocrine phenotype and growth morphology. Cancer Res. 51: 6142-6149, 1991. PubMed: 1718595 Fisher ER, Paulson JD. A new in vitro cell line established from human large cell variant of oat cell lung cancer. Cancer Res. 38: 3830-3835, 1978. PubMed: 212181 Koros AM, et al. 186Rhenium-HNK1 monoclonal antibody targets human small cell lung cancer cells in athymic nude mice: rapid screening model for therapy. Anticancer Res. 13: 1953-1956, 1993. PubMed: 7507652 Koros. Monoclonal antibodies directed against small cell carcinomas define by flow cytometry phenotypic differences among small cell and non-small cell carcinomas, neuroblastomas, and lymphoblastoid cell lines. Lung Cancer 4: 52-54, 1988.

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