| 產(chǎn)品名稱 | MyC-CaP |
|---|---|
| 商品貨號 | B161410 |
| Organism | Mus musculus, mouse |
| Tissue | prostate |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | prostate cancer |
| Age | 16 months |
| Gender | male |
| Strain | FVB; Hi-Myc transgenic |
| Applications | Study of prostate cancer progression from androgen dependence to a hormone-refractory or androgen-independent stage |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The MyC-CaP cell line was derived from a genetically engineered mouse prostate cancer removed from an animal that was never exposed to hormone ablation. |
| Receptor Expression | wild-type androgen receptor (AR) |
| Comments | The cells have an amplified androgen receptor gene despite no prior exposure to androgen withdrawal. These cells express wild-type androgen receptor (AR), retain androgen-dependent transgene expression and demonstrate androgen-dependent growth on soft agar and in mice. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Seeding Density | 5 x103 to 1 x 104 viable cells/cm2 |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: 1:5 to 1:20 is recommended. Medium renewal: 2 to 3 times a week |
| Cryopreservation | complete growth medium, 90%; DMSO, 10% |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Population Doubling Time | 18 hours |
| Name of Depositor | CL Sawyers |
| Year of Origin | 2005 |
| References | Watson PA, et al. Context-dependent hormone-refractory progression revealed through characterization of a novel murine prostate cancer cell line. Cancer Res. 65(24): 11565-11571, 2005. PubMed: 16357166 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
