| 產品名稱 | LASCPC-01 |
|---|---|
| 商品貨號 | B161409 |
| Organism | Homo sapiens, human |
| Tissue | prostate |
| Product Format | frozen 1.0 mL |
| Morphology | aggregate |
| Culture Properties | suspension/aggregate |
| Biosafety Level | 2 [Cells contain lentivirus genes]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | prostate cancer |
| Age | 75-80 |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line with the N-Myc as a driver of NEPC can serve for studying therapeutic treatments of Neuroendocrine prostate cancer (NEPC) |
| Karyotype | Conventional karyotyping: normal 46 X,Y Array CGH: mosaic gian of 1q23.1-1q44 |
| Images |  |
| Clinical Data | 75-80 years male Caucasian |
| Comments | Expression of RFP, GFP, MYCN, and myristoylated AKT1. |
| Complete Growth Medium | To prepare 500mL of complete medium:
Note: Complete culture medium is stable for 1 month once hydrocortisone and transferrin are added. |
| Subculturing | Cultures can be maintained by addition of fresh medium once or twice a week. |
| Cryopreservation | liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Cells per Vial | Since being aggregates we use 20 ml culture medium with healthy aggregates per 1 ml freeze volume |
| Volume | 1.0 mL |
| STR Profile | Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 9
TPOX: 8,11
vWA: 15,17 |
| Sterility Tests | Bacteria and yeast: No growth Mycoplasma: No growth |
| Viability | ≥ 50% |
| Population Doubling Time | Approximately 24 hours per depositor |
| Name of Depositor | J Lee |
| Year of Origin | 2014 |
| References | Lee JK, et al. N-Myc drives neuroendocrine prostate cancer initiated from human prostate epithelial cells. Canc Cell 29(4):536-47, 2016. PubMed: 27050099 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
