| 產品名稱 | BCE C/D-1b |
|---|---|
| 商品貨號 | B161386 |
| Organism | Bos taurus, cow |
| Tissue | eye, cornea |
| Cell Type | endothelial |
| Product Format | frozen |
| Morphology | endothelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Gender | male |
| Applications | The cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | This line was established from explants of normal adult bovine corneas. |
| Genes Expressed | extracellular matrix (ECM) proteins |
| Cellular Products | extracellular matrix (ECM) proteins |
| Comments | The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90. The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, add fresh trypsin (0.25%) - EDTA (0.03%) solution, rinse and remove. Add fresh trypsin solution (1 to 2 mL) and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: 1:2 to 1:4
Medium Renewal: 2 to 3 times per week
|
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | AH Nicholas, C Bethea |
| Deposited As | Bos taurus |
| Passage History | The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90. |
| References | Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215 Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
